Columbia Advanced Tissue Pathology and Imaging Core

Overview

The Advance Tissue Pathology and Imaging Core provides a spectrum of advanced cellular and tissue pathology and imaging services that meet the needs of diabetes researchers at Columbia University. The Core also provides state-of-the-art microscopy equipment and services. They include confocal, live 2-photon and scanning & transmission electron microscopy. As more advanced and specialized microscopy services have been added, the ATPI Core has added relevant technical training and provided requisite image acquisition and analysis capabilities.

Services

Pathology services

• Routine and specialized histochemical preparations and stains
• Immunohistochemistry and immunofluorescence staining of up to four colors
• Access to over 80 validated diabetes-relevant antibodies and histological reagents
• RNA in situ hybridization

Advanced microscopy services

• Standard epifluorescence
• Confocal microscopy (scanning and spinning-disk)
• Two-photon microscopy
• Wide-field fluorescence microscopy
• Super-resolution imaging (STORM and SIM)
• Cell surface imaging with <100 nM z-resolution (TIRF)
• Laser-capture microdissection and live cell imaging
• Advanced image analysis – including image deconvolution

Consultation services

• Experimental design
• Data acquisition and analysis
• Instrument training