UCSD-UCLA Genomics and Epigenetics Core

Overview

The DRC Genomics and Epigenetics Core is a state-of-the-art facility that facilitates high-throughput genomic approaches. High-throughput sequencing, data acquisition and analysis require expensive instrumentation and reagents and a highly skilled team of individuals who are experts in specific components of the overall procedure. These technologies therefore lie beyond the scope of most individual laboratories. The DRC Genomics and Epigenetics Core, which is comprised of the Institute for Genomic Medicine (IGM) and VA/VMRF Microarray & NGS Facilities at UCSD, has been instrumental in providing DRC investigators access to these technologies. Both facilities have been in operation since 1999. Their value in discovery research enables investigators to identify signal transduction pathways, uncover new targets for therapeutic intervention, and reveal biomarkers for disease detection and prognosis. Genomics services are available to the DRC community at subsidized rates.

Services

IGM Genomics Center

The UCSD IGM Genomics Center offers full services in high-throughput sequencing and whole-genome genotyping and copy number variation analysis (see Services). The IGM Genomics Center offers assay design consultation, sequencing library preparation (DNA/RNA/smallRNA/targeted sequencing), single cell RNA sequencing (10X Genomics), lllumina platform sequencing (MiSeq, HiSeq2500, HiSeq4000 and NovaSeq 6000), PacBio SMRT sequencing and Illumina genotyping and methylation arrays.

Center for Computational Biology and Bioinformatics

CCBB offers a wide variety of analytical services, including high-throughput sequencing analyses, transcriptomics, genomics, epigenomics, metagenomics, microbiome, CRISPR screens, network analysis, and custom analytical pipelines and algorithm development.

Functional Genomics Specialist

The Functional Genomics Specialist will support DRC investigators through consultation, training (both theoretical and physical) and/or collaboration as determined by contextually defined needs of individual researchers. This may include advising investigators on appropriate Genomics methods application, experimental setup and experimental planning aimed at allowing efficient advancement of their research via implementation of high-throughput sequencing.