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Multiple TOF/TOF Events in a Single Laser Shot for Multiplexed Lipid Identifications in MALDI Imaging Mass Spectrometry.
Citation | “Multiple Tof/Tof Events In A Single Laser Shot For Multiplexed Lipid Identifications In Maldi Imaging Mass Spectrometry.”. International Journal Of Mass Spectrometry, pp. 30-37. . |
Center | Vanderbilt University |
Author | Boone M Prentice, Josiah C McMillen, Richard M Caprioli |
Keywords | MALDI, MS/MS, Multiplexed, Imaging mass spectrometry, lipid |
Abstract |
Tandem mass spectrometry (MS/MS) is often used to identify lipids in matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI IMS) workflows. The molecular specificity afforded by MS/MS is crucial on MALDI time-of-flight (TOF) platforms that generally lack high resolution accurate mass measurement capabilities. Unfortunately, imaging MS/MS workflows generally only monitor a single precursor ion over the imaged area, limiting the throughput of this methodology. Herein, we demonstrate that multiple TOF/TOF events performed in each laser shot can be used to improve the throughput of imaging MS/MS. This is shown to enable the simultaneous identification of multiple phosphatidylcholine lipids in rat brain tissue. Uniquely, the separation in time achieved for the precursor ions in the TOF-1 region of the instrument is maintained for the fragment ions as they are analyzed in TOF-2, allowing for the differentiation of fragment ions of the exact same derived from different precursor ions ( the 163 fragment ion from precursor ion 772.5 is easily distinguished from the 163 fragment ion from precursor ion 826.5). This multiplexed imaging MS/MS approach allows for the acquisition of complete fragment ion spectra for multiple precursor ions per laser shot. |
Year of Publication |
2019
|
Journal |
International journal of mass spectrometry
|
Volume |
437
|
Number of Pages |
30-37
|
Date Published |
03/2019
|
ISSN Number |
1387-3806
|
DOI |
10.1016/j.ijms.2018.06.006
|
Alternate Journal |
Int J Mass Spectrom
|
PMID |
30906202
|
PMCID |
PMC6424509
|
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