Best practices and benchmarks for intact protein analysis for top-down mass spectrometry.
| Citation | . “Best Practices And Benchmarks For Intact Protein Analysis For Top-Down Mass Spectrometry.”. Nature Methods, pp. 587-594. |
| Center | UCSD-UCLA |
| Author | Daniel P Donnelly, Catherine M Rawlins, Caroline J DeHart, Luca Fornelli, Luis F Schachner, Ziqing Lin, Jennifer L Lippens, Krishna C Aluri, Richa Sarin, Bifan Chen, Carter Lantz, Wonhyeuk Jung, Kendall R Johnson, Antonius Koller, Jeremy J Wolff, Iain D G Campuzano, Jared R Auclair, Alexander R Ivanov, Julian P Whitelegge, Ljiljana Paša-Tolić, Julia Chamot-Rooke, Paul O Danis, Lloyd M Smith, Yury O Tsybin, Joseph A Loo, Ying Ge, Neil L Kelleher, Jeffrey N Agar |
| Abstract |
One gene can give rise to many functionally distinct proteoforms, each of which has a characteristic molecular mass. Top-down mass spectrometry enables the analysis of intact proteins and proteoforms. Here members of the Consortium for Top-Down Proteomics provide a decision tree that guides researchers to robust protocols for mass analysis of intact proteins (antibodies, membrane proteins and others) from mixtures of varying complexity. We also present cross-platform analytical benchmarks using a protein standard sample, to allow users to gauge their proficiency. |
| Year of Publication |
2019
|
| Journal |
Nature methods
|
| Volume |
16
|
| Issue |
7
|
| Number of Pages |
587-594
|
| Date Published |
12/2019
|
| ISSN Number |
1548-7105
|
| DOI |
10.1038/s41592-019-0457-0
|
| Alternate Journal |
Nat. Methods
|
| PMID |
31249407
|
| PMCID |
PMC6719561
|
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