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Deficient spermiogenesis in mice lacking .

Citation
Wang, F., et al. “Deficient Spermiogenesis In Mice Lacking .”. Elife.
Author Feng Wang, Maria Gracia Gervasi, Ana Bošković, Fengyun Sun, Vera D Rinaldi, Jun Yu, Mary C Wallingford, Darya A Tourzani, Jesse Mager, Lihua Julie Zhu, Oliver J Rando, Pablo E Visconti, Lara Strittmatter, Ingolf Bach
Keywords Rlim, cytoplasmic reduction, Developmental Biology, mouse, Mouse genetics, spermiogenesis
Abstract

The X-linked gene plays major roles in female mouse development and reproduction, where it is crucial for the maintenance of imprinted X chromosome inactivation in extraembryonic tissues of embryos. However, while females carrying a systemic knockout (KO) die around implantation, male KO mice appear healthy and are fertile. Here, we report an important role for in testis where it is highly expressed in post-meiotic round spermatids as well as in Sertoli cells. Systemic deletion of the gene results in lower numbers of mature sperm that contains excess cytoplasm, leading to decreased sperm motility and in vitro fertilization rates. Targeting the conditional cKO specifically to the spermatogenic cell lineage largely recapitulates this phenotype. These results reveal functions of in male reproduction specifically in round spermatids during spermiogenesis.

Year of Publication
2021
Journal
eLife
Volume
10
Date Published
02/2021
ISSN Number
2050-084X
DOI
10.7554/eLife.63556
Alternate Journal
Elife
PMID
33620316
PMCID
PMC7935487
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