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In Vivo Maturation of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes in Neonatal and Adult Rat Hearts.

Citation
Kadota, S., et al. “In Vivo Maturation Of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes In Neonatal And Adult Rat Hearts.”. Stem Cell Reports, pp. 278-289.
Center University of Washington
Author Shin Kadota, Lil Pabon, Hans Reinecke, Charles E Murry
Keywords human pluripotent stem cell-derived cardiomyocyte, maturation, neonatal rat
Abstract

We hypothesized that the neonatal rat heart would bring transplanted human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) to maturity as it grows to adult size. In neonatal rat heart, engrafted hiPSC derivatives developed partially matured myofibrils after 3 months, with increasing cell size and sarcomere length. There was no difference between grafts from hiPSC-CMs or hiPSC-derived cardiac progenitors (hiPSC-CPs) at 3 months, nor was maturation influenced by infarction. Interestingly, the infarcted adult heart induced greater human cardiomyocyte hypertrophy and induction of cardiac troponin I expression than the neonatal heart. Although human cardiomyocytes at all time points were significantly smaller than the host rat cardiomyocytes, transplanted neonatal rat cardiomyocytes reached adult size and structure by 3 months. Thus, the adult rat heart induces faster maturation than the neonatal heart, and human cardiomyocytes mature more slowly than rat cardiomyocytes. The slower maturation of human cardiomyocytes could be related to environmental mismatch or cell-autonomous factors.

Year of Publication
2017
Journal
Stem cell reports
Volume
8
Issue
2
Number of Pages
278-289
Date Published
12/2017
ISSN Number
2213-6711
DOI
10.1016/j.stemcr.2016.10.009
Alternate Journal
Stem Cell Reports
PMID
28065644
PMCID
PMC5311430
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