Differentiation and Metabolic Interrogation of Human Adipocytes.
| Citation | . “Differentiation And Metabolic Interrogation Of Human Adipocytes.”. Methods In Molecular Biology (Clifton, N.j.), pp. 61-76. |
| Center | University of Michigan |
| Author | Nicki A Baker, Lindsey A Muir, Carey N Lumeng, Robert W O'Rourke |
| Keywords | adipocyte, Adipose tissue, differentiation, Glucose uptake, lipogenesis, lipolysis, preadipocyte, Stromal vascular cell fraction |
| Abstract |
Adipocytes differentiated from preadipocytes provide a valuable model for the study of human adipocyte metabolism. We describe methods for isolation of human stromal vascular cells, expansion of preadipocytes, differentiation into mature adipocytes, and in vitro metabolic interrogation of adipocytes. |
| Year of Publication |
2017
|
| Journal |
Methods in molecular biology (Clifton, N.J.)
|
| Volume |
1566
|
| Number of Pages |
61-76
|
| Date Published |
12/2017
|
| ISSN Number |
1940-6029
|
| DOI |
10.1007/978-1-4939-6820-6_7
|
| Alternate Journal |
Methods Mol. Biol.
|
| PMID |
28244041
|
| PMCID |
PMC5762179
|
| Download citation |