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Designing 3-D Adipospheres for Quantitative Metabolic Study.
Citation | “Designing 3-D Adipospheres For Quantitative Metabolic Study.”. Methods In Molecular Biology (Clifton, N.j.), pp. 177-183. . |
Center | University of Michigan |
Author | Takeshi Akama, Brendan M Leung, Joe Labuz, Shuichi Takayama, Tae-Hwa Chun |
Keywords | adipocyte, Drop culture, mitochondria, Spheroid |
Abstract |
Quantitative assessment of adipose mitochondrial activity is critical for better understanding of adipose tissue function in obesity and diabetes. While the two-dimensional (2-D) tissue culture method has been sufficient to discover key molecules that regulate adipocyte differentiation and function, the method is insufficient to determine the role of extracellular matrix (ECM) molecules and their modifiers, such as matrix metalloproteinases (MMPs), in regulating adipocyte function in three-dimensional (3-D) in vivo-like microenvironments. By using a 3-D hanging drop tissue culture system, we are able to produce scalable 3-D adipospheres that are suitable for quantitative metabolic study in 3-D microenvironment. |
Year of Publication |
2017
|
Journal |
Methods in molecular biology (Clifton, N.J.)
|
Volume |
1566
|
Number of Pages |
177-183
|
Date Published |
12/2017
|
ISSN Number |
1940-6029
|
DOI |
10.1007/978-1-4939-6820-6_17
|
Alternate Journal |
Methods Mol. Biol.
|
PMID |
28244051
|
PMCID |
PMC5433799
|
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