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- Development of a Whole Organism Platform for Phenotype-Based Analysis of IGF1R-PI3K-Akt-Tor Action.
Development of a Whole Organism Platform for Phenotype-Based Analysis of IGF1R-PI3K-Akt-Tor Action.
Citation | “Development Of A Whole Organism Platform For Phenotype-Based Analysis Of Igf1R-Pi3K-Akt-Tor Action.”. Scientific Reports, p. 1994. . |
Center | University of Michigan |
Author | Chengdong Liu, Wei Dai, Yan Bai, Changfeng Chi, Yi Xin, Gen He, Kangsen Mai, Cunming Duan |
Abstract |
Aberrant regulation of the insulin-like growth factor (IGF)/insulin (IIS)-PI3K-AKT-TOR signaling pathway is linked to major human diseases, and key components of this pathway are targets for therapeutic intervention. Current assays are molecular target- or cell culture-based platforms. Due to the great in vivo complexities inherited in this pathway, there is an unmet need for whole organism based assays. Here we report the development of a zebrafish transgenic line, Tg(igfbp5a:GFP), which faithfully reports the mitotic action of IGF1R-PI3K-Akt-Tor signaling in epithelial cells in real-time. This platform is well suited for high-throughput assays and real-time cell cycle analysis. Using this platform, the dynamics of epithelial cell proliferation in response to low [Ca] stress and the distinct roles of Torc1 and Torc2 were elucidated. The availability of Tg(igfbp5a:GFP) line provides a whole organism platform for phenotype-based discovery of novel players and inhibitors in the IIS-PI3K-Akt-Tor signaling pathway. |
Year of Publication |
2017
|
Journal |
Scientific reports
|
Volume |
7
|
Issue |
1
|
Number of Pages |
1994
|
Date Published |
12/2017
|
ISSN Number |
2045-2322
|
DOI |
10.1038/s41598-017-01687-3
|
Alternate Journal |
Sci Rep
|
PMID |
28515443
|
PMCID |
PMC5435685
|
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