A large CRISPR-induced bystander mutation causes immune dysregulation.
| Citation | . “A Large Crispr-Induced Bystander Mutation Causes Immune Dysregulation.”. Communications Biology, p. 70. |
| Author | Dimitre R Simeonov, Alexander J Brandt, Alice Y Chan, Jessica T Cortez, Zhongmei Li, Jonathan M Woo, Youjin Lee, Claudia M B Carvalho, Alyssa C Indart, Theodore L Roth, James Zou, Andrew P May, James R Lupski, Mark S Anderson, William Buaas, Daniel S Rokhsar, Alexander Marson |
| Abstract |
A persistent concern with CRISPR-Cas9 gene editing has been the potential to generate mutations at off-target genomic sites. While CRISPR-engineering mice to delete a ~360 bp intronic enhancer, here we discovered a founder line that had marked immune dysregulation caused by a 24 kb tandem duplication of the sequence adjacent to the on-target deletion. Our results suggest unintended repair of on-target genomic cuts can cause pathogenic "bystander" mutations that escape detection by routine targeted genotyping assays. |
| Year of Publication |
2019
|
| Journal |
Communications biology
|
| Volume |
2
|
| Number of Pages |
70
|
| Date Published |
12/2019
|
| ISSN Number |
2399-3642
|
| DOI |
10.1038/s42003-019-0321-x
|
| Alternate Journal |
Commun Biol
|
| PMID |
30793048
|
| PMCID |
PMC6379443
|
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