Production of Single Contracting Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes: Matrigel Mattress Technique.
| Citation | . “Production Of Single Contracting Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes: Matrigel Mattress Technique.”. Current Protocols In Stem Cell Biology, pp. 4A.14.1-4A.14.7. |
| Center | Vanderbilt University |
| Author | Adrian G Cadar, Tromondae K Feaster, Matthew D Durbin, Charles C Hong |
| Keywords | Matrigel, Cardiac, hiPSC-CMs, myocytes, Stem cells |
| Abstract |
This unit describes the published Matrigel mattress method. Briefly, we describe the preparation of the mattress, replating of the human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) on the Matrigel mattress, and hiPSC-CM mattress maintenance. Adherence to this protocol will yield individual, robustly shortening hiPSC-CMs, which can be used for downstream applications. © 2017 by John Wiley & Sons, Inc. |
| Year of Publication |
2017
|
| Journal |
Current protocols in stem cell biology
|
| Volume |
42
|
| Number of Pages |
4A.14.1-4A.14.7
|
| Date Published |
08/2017
|
| ISSN Number |
1938-8969
|
| DOI |
10.1002/cpsc.32
|
| Alternate Journal |
Curr Protoc Stem Cell Biol
|
| PMID |
28806851
|
| PMCID |
PMC5577013
|
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