Improved Murine MHC-Deficient HLA Transgenic NOD Mouse Models for Type 1 Diabetes Therapy Development.

Improved mouse models for type 1 diabetes (T1D) therapy development are needed. T1D susceptibility is restored to normally resistant NOD.β2m mice transgenically expressing human disease-associated HLA-A*02:01 or HLA-B*39:06 class I molecules in place of their murine counterparts. T1D is dependent on pathogenic CD8 T-cell responses mediated by these human class I variants. NOD.β2m-A2.1 mice were previously used to identify β-cell autoantigens presented by this human class I variant to pathogenic CD8 T cells and for testing therapies to attenuate such effectors. However, NOD.β2m mice also lack nonclassical MHC I family members, including FcRn, required for antigen presentation, and maintenance of serum IgG and albumin, precluding therapies dependent on these molecules. Hence, we used CRISPR/Cas9 to directly ablate the NOD H2-K and H2-D classical class I variants either individually or in tandem (cMHCI). Ablation of the H2-A class II variant in the latter stock created NOD mice totally lacking in classical murine MHC expression (cMHCI/II). NOD-cMHCI mice retained nonclassical MHC I molecule expression and FcRn activity. Transgenic expression of HLA-A2 or -B39 restored pathogenic CD8 T-cell development and T1D susceptibility to NOD-cMHCI mice. These next-generation HLA-humanized NOD models may provide improved platforms for T1D therapy development.