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Postexercise improvement in glucose uptake occurs concomitant with greater γ3-AMPK activation and AS160 phosphorylation in rat skeletal muscle.

Citation
Wang, H., et al. “Postexercise Improvement In Glucose Uptake Occurs Concomitant With Greater Γ3-Ampk Activation And As160 Phosphorylation In Rat Skeletal Muscle.”. American Journal Of Physiology. Endocrinology And Metabolism, pp. E859-E871.
Center University of Michigan Joslin Diabetes Center
Multicenter
Multicenter
Author Haiyan Wang, Edward B Arias, Mark W Pataky, Laurie J Goodyear, Gregory D Cartee
Keywords AMP-activated protein kinase, TBC1D4, Exercise, glucose transport, insulin sensitivity
Abstract

A single exercise session can increase insulin-stimulated glucose uptake (GU) by skeletal muscle, concomitant with greater Akt substrate of 160 kDa (AS160) phosphorylation on Akt-phosphosites (Thr and Ser) that regulate insulin-stimulated GU. Recent research using mouse skeletal muscle suggested that ex vivo 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR) or electrically stimulated contractile activity-inducing increased γ3-AMPK activity and AS160 phosphorylation on a consensus AMPK-motif (Ser) resulted in greater AS160 Thr phosphorylation and GU by insulin-stimulated muscle. Our primary goal was to determine whether in vivo exercise that increases insulin-stimulated GU in rat skeletal muscle would also increase γ3-AMPK activity and AS160 site-selective phosphorylation (Ser, Thr, and Ser) immediately postexercise (IPEX) and/or 3 h postexercise (3hPEX). Epitrochlearis muscles isolated from sedentary and exercised (2-h swim exercise; studied IPEX and 3hPEX) rats were incubated with 2-deoxyglucose to determine GU (without insulin at IPEX; without or with insulin at 3hPEX). Muscles were also assessed for γ1-AMPK activity, γ3-AMPK activity, phosphorylated AMPK (pAMPK), and phosphorylated AS160 (pAS160). IPEX versus sedentary had greater γ3-AMPK activity, pAS160 (Ser, Thr, Ser), and GU with unaltered γ1-AMPK activity. 3hPEX versus sedentary had greater γ3-AMPK activity, pAS160 Ser, and GU with or without insulin; greater pAS160 Thr only with insulin; and unaltered γ1-AMPK activity. These results using an in vivo exercise protocol that increased insulin-stimulated GU in rat skeletal muscle are consistent with the hypothesis that in vivo exercise-induced enhancement of γ3-AMPK activation and AS160 Ser IPEX and 3hPEX are important for greater pAS160 Thr and enhanced insulin-stimulated GU by skeletal muscle.

Year of Publication
2018
Journal
American journal of physiology. Endocrinology and metabolism
Volume
315
Issue
5
Number of Pages
E859-E871
Date Published
12/2018
ISSN Number
1522-1555
DOI
10.1152/ajpendo.00020.2018
Alternate Journal
Am. J. Physiol. Endocrinol. Metab.
PMID
30130149
PMCID
PMC6293165
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