Metabolic Analysis of Lymphatic Endothelial Cells.
| Citation | . “Metabolic Analysis Of Lymphatic Endothelial Cells.”. Methods In Molecular Biology (Clifton, N.j.), pp. 325-334. |
| Center | Yale University |
| Author | Pengchun Yu, Tiago C Alves, Richard G Kibbey, Michael Simons |
| Keywords | Cellular metabolism, Fatty acid β-oxidation, Glucose oxidation, Glutamine oxidation, glycolysis, Lymphatic endothelial cells, Mass spectrometry |
| Abstract |
Metabolism is pivotal for formation of the lymphatic vasculature. Understanding metabolism in lymphatic endothelial cells (LECs) requires quantitative characterization of specific metabolic pathways. Here we describe methods for using radioactive tracers to assess flux rates of glycolysis, fatty acid β-oxidation, glucose oxidation, and glutamine oxidation. We also provide a detailed method for utilizing mass spectrometry (MS) to measure glycolytic intermediates and ATP. |
| Year of Publication |
2018
|
| Journal |
Methods in molecular biology (Clifton, N.J.)
|
| Volume |
1846
|
| Number of Pages |
325-334
|
| Date Published |
12/2018
|
| ISSN Number |
1940-6029
|
| DOI |
10.1007/978-1-4939-8712-2_22
|
| Alternate Journal |
Methods Mol. Biol.
|
| PMID |
30242770
|
| PMCID |
PMC6693514
|
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