Protein, DERC - Columbia University

DERC - Columbia Protein Production :

The protein core facility will aid DERC researchers in all aspects of protein production and characterization. Services include production of recombinant proteins in both bacterial and mammalian expression systems; protein purification; protein characterization, including assessment of protein purity, multimerization state, identity, and covalent modification. Advice on all aspects of protein chemistry and production will be available.

Services :

People :

Lawrence Shapiro, Ph.D. : Director, Protein Production Core, Columbia University, Dept. of Biochemistry and Molecular Biophysics, 630 West 168th Street, New York, NY 10032, USA. Phone: 212-342-6029

Mary Anne Gawinowicz, Ph.D. : Manager, Protein Production Core, . Phone: 212-305-3631

Protein, DRTC - University of Michigan

DRTC U Michigan Peptide and Proteomics Core :

The goal of the MDRTC Peptide and Proteomics Core is to enhance and stimulate research in diabetes, its complications and related endocrine and metabolic disorders by providing the broadest range of resources for the analysis of peptides and proteins. This core provides assistance with peptide synthesis, including multiply labeled, cyclic peptides and peptides with posttranslational modifications. 

Services :

The MDRTC Peptide and Proteomics Core provides MDRTC members with:

• Development and/or implementation of new technologies particularly beneficial to MDRTC investigators
• Access to state-of-the-art instrumentation for protein structure analysis and peptide synthesis
• Access to highly trained personnel to operate the instrumentation and interpret data, including the latest advances in protein chemistry and how their use would benefit MDRTC research programs.
• Training in the interpretation of data, operation of instruments and experimental design

The MDRTC Peptide Core is part of the existing Protein Structure Facility of the Michigan Biomedical Research Core Facilities. Technologies and techniques already being offered in the Biomedical Research Core Facilities are part of the MDRTC Core. In addition, new technologies are being developed by the MDRTC Core that specifically address needs of MDRTC investigators.

The MDRTC Core is equipped to provide a broad range of state-of-the-art services in support of basic research. Most of these services involve the use of costly instrumentation to which individual investigators would not otherwise have access to. These services also require highly trained personnel that are difficult to support by a single laboratory. Major services include:

• Peptide Synthesis Services:
  • Linear Custom Peptides
  • Peptides with Posttranslational Modifications or Unusual Amino Acids
  • Labeled Peptides
  • Peptide Libraries
  • Cyclic Peptides
  • Peptides for Immunological Applications

• Protein Mass Spectrometry Services:
  • Molecular Weight Determination of Proteins
  • Protein identification by LC-MS/MS
  • Peptide Mass Fingerprinting
  • Characterization and Identification of Posttranslational Modifications

• Other Protein Analysis Services:
  • Circular Dichroism Spectroscopy

Recharge Rates

MDRTC investigators receive a 75 percent discount on the standard recharges of the Biomedical Research Core Facilities Protein Structure Facility. Usage by MDRTC investigators will be monitored and if it appears that usage will exceed the support level, the discount rate will be changed appropriately or a cap will be placed on usage by individual investigators.

For further information, please go to the Biomedical Research Core Facilities Protein Structure Facility Web site.

People :

Philip C. Andrews, Ph.D. : Director, Peptide and Proteomics Core, . Phone: 734 763-3130

Henriette Remmer, Ph.D. : Co-Director, Peptide and Proteomics Core, . Phone: 734-763-6285