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[body] => [<div class="field field-type-text field-field-center"><div class="field-items"><div class="field-item">DERC - University of Washington</div></div></div><div class="field field-type-text field-field-objectives"><div class="field-items"><div class="field-item"><h2><span>The primary goals of the University of Washington DERC Cellular Biology Core are to: </span></h2>
<ul>
<li><span>Provide state-of-the-art cell culture facilities and technical assistance for DERC investigators, obviating duplication of these resources in their own laboratories. </span></li>
<li><span>Train DERC investigators, postdoctoral fellows, and research personnel in the design and interpretation of cell culture studies for their diabetes-related research. </span></li>
<li><span>Assist DERC investigators with developing and implementing new cell culture protocols for their diabetes research. </span></li>
</ul>
<h2><span>The Cellular Biology Core (CBC) provides DERC affiliate investigators with access to instruments, facilities, technical help, services, and collaborative expertise for research investigations based on cell culture techniques. The CBC laboratories and facilities are located in the Division of Metabolism, Endocrinology, and Nutrition, Department of Medicine, at the University of Washington School of Medicine. The CBC provides the following services: </span></h2>
<ul>
<li><span>Cells and cell cultures </span></li>
<li><span>Tissue culture equipment facility </span></li>
<li><span>Medium and buffer preparation </span></li>
<li><span>Consultation and technical assistance with experiments </span></li>
</ul>
</div></div></div><div class="field field-type-text field-field-services"><div class="field-items"><div class="field-item"><h2>Cells and Cell Cultures</h2>
<ul>
<li>The CBC has a repository of cultured cells which includes human skin fibroblasts from about 200 donors (control, type 1 diabetes, type 2 diabetes, and fetal), several islet cell lines (HIT, HIP, RIN, alpha-TC, and beta-TC), mouse myeloma cell lines NS-1 and P3X63 Ag8.653 (for hybridoma production), selected mouse cell lines (e.g., 3T3-L1), and several hybridoma cell lines. These cultures are frozen and stored in liquid nitrogen, and are provided to Affiliates either frozen or after being reinitiated into culture.</li>
<li>The CBC initiates fibroblast cultures from skin biopsies and other types of tissues provided by Affiliates. Examples include newborn rat pancreas (islet monolayer cultures) and human islet cell tumors. The CBC will immortalize peripheral blood human lymphocytes by E-B virus transformation. Transformed cells may be either stored in the CBC or transferred to the Affiliate. The DERC <a href="http://depts.washington.edu/diabetes/biomedcore/ifac.index.html">Islet Cell and Functional Analysis Core</a> provides isolated islets from several species.</li>
<li>In order to obtain cultures from the CBC, Affiliates should indicate the type and number of cultures desired, a brief summary of the project in which the culture will be used, and funding support for the project.</li>
</ul>
<h2>Tissue Culture Equipment</h2>
<ul>
<li>The CBC provides access to some equipment, including biological safety cabinets, tissue culture incubators, phase contrast microscope (with photomicroscopic and fluorescence microscopy capability), and other specialized instruments for cell and tissue culture. These facilities are located at the University of Washington School of Medicine.</li>
</ul>
<h2>Medium and Buffer Preparation</h2>
<ul>
<li>The CBC prepares a variety of culture media, buffers, and balanced salt solutions. All solutions are sterility-tested prior to release from the facility.</li>
<li>Affiliates who wish to take advantage of this service should submit a brief summary of their project and funding support for the project, and indicate the type and amounts of solution they require. If this is a "custom" solution, then the directions for preparation should also be submitted. Affiliates must provide the CBC with bottles and the powder base for media, as well as any special additives. The CBC will supply those salts commonly used for constructing buffers and salt solutions.</li>
</ul>
<h2>Consultation and Technical Assistance</h2>
<p>Consultation concerning tissue culture is available from the CBC. There is no charge for this service. In addition, the CBC provides technical assistance to Affiliates for running experiments in the CBC. These include assistance with the growing of hybridoma cultures provided by Affiliates or from the CBC repository, performance of both static and perifusion incubations, hormone (insulin and glucagon) radioimmunoassays of both incubation media and cell (tissue) extracts, and DNA measurements of cell extracts. When experiments are done in the CBC, data analysis and summarization will also be provided.</p>
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[value] => [<h2><span style="font-size: small">The primary goals of the University of Washington DERC Cellular Biology Core are to: </span></h2><ul><li><span style="font-size: small">Provide state-of-the-art cell culture facilities and technical assistance for DERC investigators, obviating duplication of these resources in their own laboratories. </span></li><li><span style="font-size: small">Train DERC investigators, postdoctoral fellows, and research personnel in the design and interpretation of cell culture studies for their diabetes-related research. </span></li><li><span style="font-size: small">Assist DERC investigators with developing and implementing new cell culture protocols for their diabetes research. </span></li></ul><h2><span style="font-size: small">The Cellular Biology Core (CBC) provides DERC affiliate investigators with access to instruments, facilities, technical help, services, and collaborative expertise for research investigations based on cell culture techniques. The CBC laboratories and facilities are located in the Division of Metabolism, Endocrinology, and Nutrition, Department of Medicine, at the University of Washington School of Medicine. The CBC provides the following services: </span></h2><ul><li><span style="font-size: small">Cells and cell cultures </span></li><li><span style="font-size: small">Tissue culture equipment facility </span></li><li><span style="font-size: small">Medium and buffer preparation </span></li><li><span style="font-size: small">Consultation and technical assistance with experiments </span></li></ul>]
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<ul>
<li><span>Provide state-of-the-art cell culture facilities and technical assistance for DERC investigators, obviating duplication of these resources in their own laboratories. </span></li>
<li><span>Train DERC investigators, postdoctoral fellows, and research personnel in the design and interpretation of cell culture studies for their diabetes-related research. </span></li>
<li><span>Assist DERC investigators with developing and implementing new cell culture protocols for their diabetes research. </span></li>
</ul>
<h2><span>The Cellular Biology Core (CBC) provides DERC affiliate investigators with access to instruments, facilities, technical help, services, and collaborative expertise for research investigations based on cell culture techniques. The CBC laboratories and facilities are located in the Division of Metabolism, Endocrinology, and Nutrition, Department of Medicine, at the University of Washington School of Medicine. The CBC provides the following services: </span></h2>
<ul>
<li><span>Cells and cell cultures </span></li>
<li><span>Tissue culture equipment facility </span></li>
<li><span>Medium and buffer preparation </span></li>
<li><span>Consultation and technical assistance with experiments </span></li>
</ul>
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[value] => [<h2>Cells and Cell Cultures</h2><ul><li>The CBC has a repository of cultured cells which includes human skin fibroblasts from about 200 donors (control, type 1 diabetes, type 2 diabetes, and fetal), several islet cell lines (HIT, HIP, RIN, alpha-TC, and beta-TC), mouse myeloma cell lines NS-1 and P3X63 Ag8.653 (for hybridoma production), selected mouse cell lines (e.g., 3T3-L1), and several hybridoma cell lines. These cultures are frozen and stored in liquid nitrogen, and are provided to Affiliates either frozen or after being reinitiated into culture.</li><li>The CBC initiates fibroblast cultures from skin biopsies and other types of tissues provided by Affiliates. Examples include newborn rat pancreas (islet monolayer cultures) and human islet cell tumors. The CBC will immortalize peripheral blood human lymphocytes by E-B virus transformation. Transformed cells may be either stored in the CBC or transferred to the Affiliate. The DERC <a href="http://depts.washington.edu/diabetes/biomedcore/ifac.index.html">Islet Cell and Functional Analysis Core</a> provides isolated islets from several species.</li><li>In order to obtain cultures from the CBC, Affiliates should indicate the type and number of cultures desired, a brief summary of the project in which the culture will be used, and funding support for the project.</li></ul><h2>Tissue Culture Equipment</h2><ul><li>The CBC provides access to some equipment, including biological safety cabinets, tissue culture incubators, phase contrast microscope (with photomicroscopic and fluorescence microscopy capability), and other specialized instruments for cell and tissue culture. These facilities are located at the University of Washington School of Medicine.</li></ul><h2>Medium and Buffer Preparation</h2><ul><li>The CBC prepares a variety of culture media, buffers, and balanced salt solutions. All solutions are sterility-tested prior to release from the facility.</li><li>Affiliates who wish to take advantage of this service should submit a brief summary of their project and funding support for the project, and indicate the type and amounts of solution they require. If this is a "custom" solution, then the directions for preparation should also be submitted. Affiliates must provide the CBC with bottles and the powder base for media, as well as any special additives. The CBC will supply those salts commonly used for constructing buffers and salt solutions.</li></ul><h2>Consultation and Technical Assistance</h2><p>Consultation concerning tissue culture is available from the CBC. There is no charge for this service. In addition, the CBC provides technical assistance to Affiliates for running experiments in the CBC. These include assistance with the growing of hybridoma cultures provided by Affiliates or from the CBC repository, performance of both static and perifusion incubations, hormone (insulin and glucagon) radioimmunoassays of both incubation media and cell (tissue) extracts, and DNA measurements of cell extracts. When experiments are done in the CBC, data analysis and summarization will also be provided.</p>]
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<ul>
<li>The CBC has a repository of cultured cells which includes human skin fibroblasts from about 200 donors (control, type 1 diabetes, type 2 diabetes, and fetal), several islet cell lines (HIT, HIP, RIN, alpha-TC, and beta-TC), mouse myeloma cell lines NS-1 and P3X63 Ag8.653 (for hybridoma production), selected mouse cell lines (e.g., 3T3-L1), and several hybridoma cell lines. These cultures are frozen and stored in liquid nitrogen, and are provided to Affiliates either frozen or after being reinitiated into culture.</li>
<li>The CBC initiates fibroblast cultures from skin biopsies and other types of tissues provided by Affiliates. Examples include newborn rat pancreas (islet monolayer cultures) and human islet cell tumors. The CBC will immortalize peripheral blood human lymphocytes by E-B virus transformation. Transformed cells may be either stored in the CBC or transferred to the Affiliate. The DERC <a href="http://depts.washington.edu/diabetes/biomedcore/ifac.index.html">Islet Cell and Functional Analysis Core</a> provides isolated islets from several species.</li>
<li>In order to obtain cultures from the CBC, Affiliates should indicate the type and number of cultures desired, a brief summary of the project in which the culture will be used, and funding support for the project.</li>
</ul>
<h2>Tissue Culture Equipment</h2>
<ul>
<li>The CBC provides access to some equipment, including biological safety cabinets, tissue culture incubators, phase contrast microscope (with photomicroscopic and fluorescence microscopy capability), and other specialized instruments for cell and tissue culture. These facilities are located at the University of Washington School of Medicine.</li>
</ul>
<h2>Medium and Buffer Preparation</h2>
<ul>
<li>The CBC prepares a variety of culture media, buffers, and balanced salt solutions. All solutions are sterility-tested prior to release from the facility.</li>
<li>Affiliates who wish to take advantage of this service should submit a brief summary of their project and funding support for the project, and indicate the type and amounts of solution they require. If this is a "custom" solution, then the directions for preparation should also be submitted. Affiliates must provide the CBC with bottles and the powder base for media, as well as any special additives. The CBC will supply those salts commonly used for constructing buffers and salt solutions.</li>
</ul>
<h2>Consultation and Technical Assistance</h2>
<p>Consultation concerning tissue culture is available from the CBC. There is no charge for this service. In addition, the CBC provides technical assistance to Affiliates for running experiments in the CBC. These include assistance with the growing of hybridoma cultures provided by Affiliates or from the CBC repository, performance of both static and perifusion incubations, hormone (insulin and glucagon) radioimmunoassays of both incubation media and cell (tissue) extracts, and DNA measurements of cell extracts. When experiments are done in the CBC, data analysis and summarization will also be provided.</p>
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<ul>
<li><span>Provide state-of-the-art cell culture facilities and technical assistance for DERC investigators, obviating duplication of these resources in their own laboratories. </span></li>
<li><span>Train DERC investigators, postdoctoral fellows, and research personnel in the design and interpretation of cell culture studies for their diabetes-related research. </span></li>
<li><span>Assist DERC investigators with developing and implementing new cell culture protocols for their diabetes research. </span></li>
</ul>
<h2><span>The Cellular Biology Core (CBC) provides DERC affiliate investigators with access to instruments, facilities, technical help, services, and collaborative expertise for research investigations based on cell culture techniques. The CBC laboratories and facilities are located in the Division of Metabolism, Endocrinology, and Nutrition, Department of Medicine, at the University of Washington School of Medicine. The CBC provides the following services: </span></h2>
<ul>
<li><span>Cells and cell cultures </span></li>
<li><span>Tissue culture equipment facility </span></li>
<li><span>Medium and buffer preparation </span></li>
<li><span>Consultation and technical assistance with experiments </span></li>
</ul>
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[#value] => [<div class="field field-type-text field-field-services"><div class="field-items"><div class="field-item"><h2>Cells and Cell Cultures</h2>
<ul>
<li>The CBC has a repository of cultured cells which includes human skin fibroblasts from about 200 donors (control, type 1 diabetes, type 2 diabetes, and fetal), several islet cell lines (HIT, HIP, RIN, alpha-TC, and beta-TC), mouse myeloma cell lines NS-1 and P3X63 Ag8.653 (for hybridoma production), selected mouse cell lines (e.g., 3T3-L1), and several hybridoma cell lines. These cultures are frozen and stored in liquid nitrogen, and are provided to Affiliates either frozen or after being reinitiated into culture.</li>
<li>The CBC initiates fibroblast cultures from skin biopsies and other types of tissues provided by Affiliates. Examples include newborn rat pancreas (islet monolayer cultures) and human islet cell tumors. The CBC will immortalize peripheral blood human lymphocytes by E-B virus transformation. Transformed cells may be either stored in the CBC or transferred to the Affiliate. The DERC <a href="http://depts.washington.edu/diabetes/biomedcore/ifac.index.html">Islet Cell and Functional Analysis Core</a> provides isolated islets from several species.</li>
<li>In order to obtain cultures from the CBC, Affiliates should indicate the type and number of cultures desired, a brief summary of the project in which the culture will be used, and funding support for the project.</li>
</ul>
<h2>Tissue Culture Equipment</h2>
<ul>
<li>The CBC provides access to some equipment, including biological safety cabinets, tissue culture incubators, phase contrast microscope (with photomicroscopic and fluorescence microscopy capability), and other specialized instruments for cell and tissue culture. These facilities are located at the University of Washington School of Medicine.</li>
</ul>
<h2>Medium and Buffer Preparation</h2>
<ul>
<li>The CBC prepares a variety of culture media, buffers, and balanced salt solutions. All solutions are sterility-tested prior to release from the facility.</li>
<li>Affiliates who wish to take advantage of this service should submit a brief summary of their project and funding support for the project, and indicate the type and amounts of solution they require. If this is a "custom" solution, then the directions for preparation should also be submitted. Affiliates must provide the CBC with bottles and the powder base for media, as well as any special additives. The CBC will supply those salts commonly used for constructing buffers and salt solutions.</li>
</ul>
<h2>Consultation and Technical Assistance</h2>
<p>Consultation concerning tissue culture is available from the CBC. There is no charge for this service. In addition, the CBC provides technical assistance to Affiliates for running experiments in the CBC. These include assistance with the growing of hybridoma cultures provided by Affiliates or from the CBC repository, performance of both static and perifusion incubations, hormone (insulin and glucagon) radioimmunoassays of both incubation media and cell (tissue) extracts, and DNA measurements of cell extracts. When experiments are done in the CBC, data analysis and summarization will also be provided.</p>
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[#children] => [<div class="field field-type-text field-field-center"><div class="field-items"><div class="field-item">DERC - University of Washington</div></div></div><div class="field field-type-text field-field-objectives"><div class="field-items"><div class="field-item"><h2><span>The primary goals of the University of Washington DERC Cellular Biology Core are to: </span></h2>
<ul>
<li><span>Provide state-of-the-art cell culture facilities and technical assistance for DERC investigators, obviating duplication of these resources in their own laboratories. </span></li>
<li><span>Train DERC investigators, postdoctoral fellows, and research personnel in the design and interpretation of cell culture studies for their diabetes-related research. </span></li>
<li><span>Assist DERC investigators with developing and implementing new cell culture protocols for their diabetes research. </span></li>
</ul>
<h2><span>The Cellular Biology Core (CBC) provides DERC affiliate investigators with access to instruments, facilities, technical help, services, and collaborative expertise for research investigations based on cell culture techniques. The CBC laboratories and facilities are located in the Division of Metabolism, Endocrinology, and Nutrition, Department of Medicine, at the University of Washington School of Medicine. The CBC provides the following services: </span></h2>
<ul>
<li><span>Cells and cell cultures </span></li>
<li><span>Tissue culture equipment facility </span></li>
<li><span>Medium and buffer preparation </span></li>
<li><span>Consultation and technical assistance with experiments </span></li>
</ul>
</div></div></div><div class="field field-type-text field-field-services"><div class="field-items"><div class="field-item"><h2>Cells and Cell Cultures</h2>
<ul>
<li>The CBC has a repository of cultured cells which includes human skin fibroblasts from about 200 donors (control, type 1 diabetes, type 2 diabetes, and fetal), several islet cell lines (HIT, HIP, RIN, alpha-TC, and beta-TC), mouse myeloma cell lines NS-1 and P3X63 Ag8.653 (for hybridoma production), selected mouse cell lines (e.g., 3T3-L1), and several hybridoma cell lines. These cultures are frozen and stored in liquid nitrogen, and are provided to Affiliates either frozen or after being reinitiated into culture.</li>
<li>The CBC initiates fibroblast cultures from skin biopsies and other types of tissues provided by Affiliates. Examples include newborn rat pancreas (islet monolayer cultures) and human islet cell tumors. The CBC will immortalize peripheral blood human lymphocytes by E-B virus transformation. Transformed cells may be either stored in the CBC or transferred to the Affiliate. The DERC <a href="http://depts.washington.edu/diabetes/biomedcore/ifac.index.html">Islet Cell and Functional Analysis Core</a> provides isolated islets from several species.</li>
<li>In order to obtain cultures from the CBC, Affiliates should indicate the type and number of cultures desired, a brief summary of the project in which the culture will be used, and funding support for the project.</li>
</ul>
<h2>Tissue Culture Equipment</h2>
<ul>
<li>The CBC provides access to some equipment, including biological safety cabinets, tissue culture incubators, phase contrast microscope (with photomicroscopic and fluorescence microscopy capability), and other specialized instruments for cell and tissue culture. These facilities are located at the University of Washington School of Medicine.</li>
</ul>
<h2>Medium and Buffer Preparation</h2>
<ul>
<li>The CBC prepares a variety of culture media, buffers, and balanced salt solutions. All solutions are sterility-tested prior to release from the facility.</li>
<li>Affiliates who wish to take advantage of this service should submit a brief summary of their project and funding support for the project, and indicate the type and amounts of solution they require. If this is a "custom" solution, then the directions for preparation should also be submitted. Affiliates must provide the CBC with bottles and the powder base for media, as well as any special additives. The CBC will supply those salts commonly used for constructing buffers and salt solutions.</li>
</ul>
<h2>Consultation and Technical Assistance</h2>
<p>Consultation concerning tissue culture is available from the CBC. There is no charge for this service. In addition, the CBC provides technical assistance to Affiliates for running experiments in the CBC. These include assistance with the growing of hybridoma cultures provided by Affiliates or from the CBC repository, performance of both static and perifusion incubations, hormone (insulin and glucagon) radioimmunoassays of both incubation media and cell (tissue) extracts, and DNA measurements of cell extracts. When experiments are done in the CBC, data analysis and summarization will also be provided.</p>
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