Cell Biology & Image Analysis, DERC - University of Colorado

DERC University of Colorado Histology & Flow Cytometry Core :

The DERC Histology & Flow Cytometry Core provides users with access to light microscopy technologies

Services :

Routine histological services in tissue fixation, processing, sectioning and staining with histochemical dyes and immunochemical reagents. Provision of slide and tissue banks of commonly used tissues or animal models e.g. developmental or NOD disease progression.

Epifluorescence microscopy for acquisition of images and performance of morphometric analyses.

Confocal microscopy for use by investigators interested in high-resolution fluorescence microscopy and live cell imaging requiring the use of FRAP and FRET analyses.

In-situ hybridization analyses that include all elements from acquisition and amplification of the gene of interest, probe synthesis, optimization of hybridization conditions and performance of multigene analyses on a moderate scale.

Higher-level microscopy including access to instrumentation and services in Laser Capture Microscopy, 2-photon confocal microscopy, spinning disk confocal microscopy, Total Internal Reflectance microscopy, conventional and immunoelectron microscopy.

Flow cytometry within the Core and provision of cell-sorting capabilities and higher level flow cytometry through outsourcing.

Training of fellows and students in morphological and morphometric techniques and consultation in specific areas including microdissection and single cell microinjection, low-level computer programming.

People :

Dirk Homann, Ph.D. : Director, Flow Cytomerty Core, Barbara Davis Center for Childhood Diabetes, UCDHSC. Assistant Professor, Departments of Pediatrics and Preventive Medicine & Biometrics. Phone: 303-724-6843

Roberto Gianani, M.D. : Director, Histology Core, Barbara Davis Center for Childhood Diabetes, UCDHSC. Assistent Professor, Department of Pediatrics. Phone: 303-724-6842